Every semester, EES organise a seminar series, which takes place on Mondays at 16:00 at Biozentrum, Großhaderner Str. 2, 82152 Planegg-Martinsried, Lecture Hall B 01.027
Speakers are invited from mainly around Europe across all three areas (Evolution, Ecology and Systematics), leading to a wide-range of presented research topics.
Winter Semester 2018/2019
David Garfield - Single-cell and population genetic approaches to understand developmental evolution
Humboldt University, Berlin, Germany
Please note: This seminar will exceptionally take place on a Wednesday at 13:00 h!
Abstract: Mutations affecting non-coding, regulatory DNA play an important role in evolution and contribute disproportionately to human disease phenotypes. But identifying functional mutations in non-coding DNA is hard – typically we don’t know where to look, and when we do, we often find redundancy and degeneracy that complicate a simple mapping of genotype to phenotype. New advances in single-cell sequencing technologies, particularly single-cell ATAC-Seq, can help to delineate and identify regulatory elements active in different tissues. We recently used one such approach to construct a cell-type specific atlas of regulatory DNA used during embryonic development in Drosophila. In this talk, I will discuss our efforts to move this atlas one step farther, using population genetic information and allele-specific sequencing to understand how the impacts of selection and functional mutations are distributed across this regulatory landscape, with an eye towards the role that single-cell assays and evolutionary analyses can play in understanding the evolution of development across a range of taxa.
Fritz Sedlazeck - Size matters: accurate detection and phasing of structural variations
Baylor University, Texas, USA
Abstract: In this presentation I will describe our latest work to obtain comprehensive genomes leveraging long and linked reads. The vast majority of NGS whole-genome data covers hundreds of thousands of samples with short illumina reads, which are unable to capture the full spectrum of genetic variation and genomic complexity. Such comprehensive variation is critical to understanding the full heritability and genetic foundations of human disease. In this seminar I will present our novel alignment strategy (NGMLR) for long read data (Oxford Nanopore and PacBio) and our novel Structural Variations (SVs) caller Sniffles. These two methods improved the accuracy for both technologies enabling the accurate and easy detection of SVs. This includes also nested events that we have previously been blind to or linked events connecting genes over multiple regions. We will discuss problems, characteristics and limitations of short reads. In addition, I will discuss the impact of these novel found SVs in cancer and other genomes with respect to RNA seq. In the end, I will highlight our current findings where we combine these long read technologies with linked reads to be able to phase SNV and SVs together to obtain a diploid genome per sample. These phased genomes are the most comprehensive representation of genomes up to date and we can now finally generate them within days.
Kees van Oers -
NICO, The Netherlands
Markus Knaden - Olfactory-guided navigation in a desert ant
Max Planck Institute for Chemical Ecology, Jena, Germany
Krysztof Kozak -
Smithsonian Tropical Research Institute, Panama
Simone Immer -
University of East Anglia, UK
Sonja Grath -
LMU Munich, Germany
Konrad Lohse -
University of Edinburgh, UK
Summer Semester 2018
Martin Kapun - Clines and inversions as evidence for local adaptation in Drosophila melanogaster
University of Fribourg, Switzerland
Abstract: Clines, which are gradual changes of genotypes or phenotypes along environmental transects, are often taken as prima facie evidence for the action of spatially varying selection. However, only due to recent advances in sequencing technology it now becomes possible to compare genome-wide clinal patterns and test for alternative models. We use the Drosophila melanogaster system to study genomic diversity along a latitudinal temperature gradient at the North American east coast. We found evidence for steep and temporally stable clinal variation associated with In(3R)Payne, a common cosmopolitan inversion, that cannot be explained by demography alone. To learn more about the potential adaptive effect of this inversion, we compare karyotype-specific genomic and transcriptomic variation on different continents. We find genomic regions in the center of the inversion that are in strong linkage disequilibrium with the inversion breakpoints possibly as a result of selection for inversion-specific genetic variation. Finally, with the help of a newly founded population genetics consortium, we now expand the genome-wide analysis of clinal genomic variation to the yet largely unexplored European continent and find further evidence for the clinal variation of In(3R)Payne despite complex demographic patterns.
Dieter Heylen - Evolutionary ecological interactions between songbirds, ticks and Borrelia burgdorferi s.l.: a community perspective
University of Antwerp, Belgium
Please note that this seminar will Mutations affecting non-coding, regulatory DNA play an important role in evolution and contribute disproportionately to human disease phenotypes. But identifying functional mutations in non-coding DNA is hard – typically we don’t know where to look, and when we do, we often find redundancy and degeneracy that complicate a simple mapping of genotype to phenotype. New advances in single-cell sequencing technologies, particularly single-cell ATAC-Seq, can help to delineate and identify regulatory elements active in different tissues. We recently used one such approach to construct a cell-type specific atlas of regulatory DNA used during embryonic development in Drosophila. In this talk, I will discuss our efforts to move this atlas one step farther, using population genetic information and allele-specific sequencing to understand how the impacts of selection and functional mutations are distributed across this regulatory landscape, with an eye towards the role that single-cell assays and evolutionary analyses can play in understanding the evolution of development across a range of taxa.
Abstract: Dieter Heylen is postdoctoral at the University of Antwerp (Biology department, Evolutionary Ecology group). He has more than ten years of experience in the study of ticks, songbirds and tick-borne diseases (TBD). He studies how the evolutionary ecology of host-parasites interactions relates to virulence, ecological specialization, adaptation, and spatio-temporal occurrence of ticks and TBD in songbirds. In more recent work, he investigates how ticks and birds contribute in the maintenance and establishment of TBD foci, by studying the reservoir competence of songbird species, and the vector-competence of bird-specialized tick species. He currently investigates how landscape determines TBD transmission flows over urbanisation gradients, and how isotope ratios of ticks can help in
elucidating transmission dynamics in the wild. In his seminar, he will consider the findings on the ecological interactions between ixodid ticks, common European songbirds and the Lyme disease causing bacteria Borrelia burgdorferi s.l..
Chris Jiggins - Understanding porous species boundaries by studying butterfly genomes
University of Cambridge, UK
Abstract: A major undertaking in evolutionary biology is to link genotype to phenotype and understand the evolutionary changes that lead to adaptation and speciation. Here I will give an overview of our work on the brightly coloured Heliconius butterflies, showing that hybridisation makes species boundaries remarkably porous across the genome. Patterns of species relationships are highly variable across the genome, and this can offer insight into the process of speciation. There is little evidence for changes in recombination rate or inversion differences between species during speciation. Despite these loci of large effect, across the genome there is evidence for pervasive polygenic selection maintaining species differences in the face of ongoing gene flow, indicated by a strong association of admixture with regions of high recombination.
Gergely Szollosi - Gene transfers, like fossils, can date the tree of life
University of Budapest, Hungary
Abstract: The geological record provides the only source of absolute time information to date the tree of life. But most life is microbial, and most microbes do not fossilize, leading to major uncertainties about the ages of microbial groups and the timing of some of the earliest and most important events in life's evolutionary history. I discuss our recent results, which show that patterns of lateral gene transfer deduced from analysis of modern genomes encode a novel and highly informative source of information about the temporal coexistence of lineages throughout the history of life. We use new phylogenetic methods to reconstruct the history of thousands of gene families and show that dates implied by gene transfers are strongly correlated with estimates from relaxed molecular clocks in Bacteria, Archaea and Eukaryotes. A comparison with mammalian fossils shows that gene transfer in microbes is potentially as informative for dating the tree of life as the geological record in macroorganisms.
Rodrigo Medellin - How to do conservation science, implement it, and not die trying.
Instituto de Ecología, UNAM, Mexico
Abstract: Conducting research for conservation is, unfortunately, too often cut short or rarely implemented. I will discuss a few examples of research projects that have become official federal government programs with nation-wide implications. Mexico is the fifth country with the greatest biodiversity in the world. Challenges are thick and plentiful. Recently Mexico became the first country in the world to have an estimate of how many jaguars inhabit the country and the National Jaguar Strategy is fully in place and being implemented today. I will also speak about bighorn sheep and how a sustainable harvest has become the heart and soul of a strong conservation and development program for the Seri indigenous group. Bats represent about one-fourth of Mexico’s mammals and they include critically endangered and endangered species. The lesser long-nosed bat has been a focal species for my research and after 25 years it was recently delisted from Mexico’s Endangered Species List. The recovery implied lots of research, education, and specific conservation actions. The job of conservation professionals must include working with government and public to be effective.
Tuncay Baubec - Function and regulation of mammalian DNA methylation
University of Zurich, Switzerland
Abstract: DNA methylation is a prevalent epigenetic modification involved in transcriptional regulation and essential for mammalian development. In mammalian genomes, DNA methylation is a prevalent modification that decorates the majority of cytosines. It is found at the promoters and enhancers of inactive genes, at repetitive elements, and within transcribed gene bodies. Its presence at promoters is dynamically linked to gene activity, suggesting that it could directly influence gene expression patterns and cellular identity. While the genome-wide distribution of this mark has been studied to great detail, the mechanisms responsible for its correct deposition, as well as the cause for its aberrant localization in cancers, have not been fully elucidated. I will present our recent efforts to elucidate the targeting preferences of DNA methyltransferases to the genome, and how chromatin states, histone modifications and DNA sequence help to guide deposition of DNA methylation to specific genomic sites.
Homa Papoli - The evolution of sex chromosomes and sex-linked sequences in systems with female heterogamety
Uppsala University, Sweden
Abstract: Sex chromosomes evolved from a pair of autosomes between which recombination stopped. We can look at sex chromosome evolution at two levels. The first is looking at a large evolutionary time scale to investigate the steps involved in recombination cessation. In another level, we can look at the consequences of recombination cessation in the evolution of sex linked sequences. We used ostrich, as a representative of a basal clade of birds, together with several avian species to infer the chromosomal inversions involved in the evolution of sex chromosomes. To understand the consequences of recombination suppression on the evolution of DNA sequences, we used multiple avian species and a population level data of ostrich sex chromosomes to infer determinants of genetic diversity.
Thomas Gilbert - Dogs and Wolves in Time and Space
University of Copenhagen, Denmark
Abstract: Despite the key position that dogs hold in the lives of many of us, and the extensive efforts of many previous scientific studies, a surprising amount remains to be learnt about our faithful friends. For example, considerable controversy exists over such basic questions as: When did we first domesticate the dog? Where was the domestication centre? Was there more than one? And perhaps most surprisingly, what was the dog even domesticated from? Given the extent to which we have both moved and shaped dog breeds in recent centuries, and taken a good stab at eradicating their wild relatives, it seems unlikely that analyses of modern genetic material alone will be able to solve these questions. As such deciphering dog domestication represents an exciting frontier on which palaeogenomic approaches stand to make enormous contribution, and indeed, in light of a vastly expanded reference dataset of contemorary genomic material, a number of intriguing findings are already coming to light.